Sodium boric acid
From Biocrawler, the free encyclopedia.
Sodium boric acid is used in the laboratory as buffer for gel electrophoresis of DNA. It has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-35V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower then with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected with sodium boric acid gels.
Lithium boric acid is similar to sodium boric acid and has all of its advantages, but permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions.
Source: Analytical Biochemistry 2004; 333: 1-13.
 | This biology article is a stub. You can help Biocrawler by expanding it (http://www.biocrawler.com/w/index.php?title=Sodium_boric_acid&action=edit). |
