His-tag
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Structure
A his-tag consists of approximately six histidine residues near the N- or C-Terminus of a Protein. The total number of histidine residues may vary. It may also be followed by a suitable amino acid sequence that facilitates a removal of the his-tag.
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Purpose
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Purification
The his-tag can be used in affinity chromatography together with a column that has nickel bound to it. Histidine is a good ligand for nickel so the fusion protein will bind to the column. The protein can be eluted with a solution of imidazole.
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Detection
The his tag can also be used in detection of the protein via anti-his-tag antibodies. This can be useful in localization, ELISA, western blots or other immunoanalytical methods.
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