Differential centrifugation

From Biocrawler, the free encyclopedia.

Differential(Rate Zonal) centrifugation is a procedure in which the homogenate is subjected to repeated centrifugations each time increasing the centrifugal force. -Uses equilibrium density gradient: A solution composed of different layers because of the difference in density between the layers. Differential centrifugation separates the homogenate into:

1. Nuclei
2. Mitochondria
3. Lysosomes
4. Microsomes
5. Soluble fraction

A homogenization solution is used to acquire the desired separation of homogenate.

Wikipedia (http://en.wikipedia.org/wiki/Main_Page) Differential_centrifugation (http://en.wikipedia.org/wiki/Differential_centrifugation) version history (http://en.wikipedia.org/w/index.php?title=Differential_centrifugation&action=history) GNU Free Documentation Lizenz (http://en.wikipedia.org/wiki/Wikipedia:Text_of_the_GNU_Free_Documentation_License) CC-by-sa (http://creativecommons.org/licenses/by-sa/2.5/)

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